The southern gate is simultaneously constructed with the Tiepolo bastion during the first phase of the fortress construction (1579-1584). Partially carved into the natural rock, it has the form of a vaulted portico with a length of 21.00 meters and has eight reinforcing arches made of limestone. Initially, its height was greater, with stepped access to the interior with stone landings. During the Leper Colony period, its interior is covered with cement. Based on the iron hinges (door mountings) preserved at the entrance, it is concluded that there were two successive doors.

During the Venetian period, the gate had an auxiliary character. In Ottoman times, it provides access to the customs building and the other structures of the southern dock. At the same time, during the Leper colony period it leads to the Court of Misdemeanors and the patients' recreation area.

This gate was used more than the main western gate, as access to the southern dock is easier due to the winds. Today, it serves as the main entrance for visitors, who are attracted by its small size and the darkness that draws them to the fortress's interior.